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Practical considerations for stem cell based predictive assays
18 February 2009
Practical considerations for stem cell based predictive assays
Overview
The first two sessions of the workshop will consist of plenary sessions with introductory presentations, which are open for discussion. After a summary of how stem cells are used in predictive toxicology, the assays will be put into perspective to other in vitro models. Discussions shall further include regulatory aspects. In the second part of the workshop practical issues will be presented and discussed. Experience exchange will be further facilitated. Particularly, questions and problems from the auditorium will be addressed during the third session. Each participant shall bring a poster presenting his current work and problems, which will be viewed by the other participants during the coffee break. The third session will be held as an open discussion forum, where participants will have the opportunity to discuss their posters and potential challenges. The goal of this workshop is to convene users of stem cell models of developmental toxicity to share their experiences, so that learning curves can be shortened.

8:30      Registration and Coffee
9:00      Welcome and introductions
9:10      Session 1 (Overview on stem cell based predictive assays)
  • Stem cells for toxicity testing – an overview
  • Comparing stem cell assays with other in vitro models
  • Caveats and recent improvements of stem cell assays
  • Legislation, regulation
10:00    Session 2 (Best practice)
  • choice of cell species
  • standardization of stem cell culture
  • pluripotency
  • serum-free versus serum-containing cultures
  • choosing an assay endpoint
10:50    Morning Coffee (Poster viewing)
11:20    Session 3 (Discussion)
12:10    Summary
12:30    Close of Workshop

Dr. Nicole zur Nieden has 10 years of experience with stem cells and stem cell-based assays. Currently, she holds a double appointment as a Research Group Leader with the Fraunhofer Institute in Leipzig, Germany and as an Assistant Professor at the University of California, Riverside, USA. She works on the large-scale expansion and differentiation of embryonic stem cells, the role of signalling pathways involved in skeletal development and the application of stem cells in toxicology prediction.

The Fraunhofer Institutes are a society of 58 non-profit research institutes with over 40 different locations throughout Germany. Fraunhofer has a unique business model that encourages industry partnership; it facilitates application of scientific results directly in industry in the development of marketable scientific concepts, encouragement of patent applications and mentoring in entrepreneurship. The objective of the Fraunhofer Institute for Immunology and Cell Therapy (IZI) core competencies are to be found in immunology and regenerative medicine, or more precisely in cell-therapeutic methods of regenerating non-functioning tissue and organs through to the biological substitution with tissue cultivated in vitro.

Workshop agenda

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8:30

Registration & Coffee

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9:00

Chairman's Opening Remarks

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9:10

Overview on stem cell based predictive assays

Nicole zur Nieden

Nicole zur Nieden, Research Group Leader , Fraunhofer Institute For Cell Therapy & Immunology

  • Stem cells for toxicity testing – an overview
  • Comparing stem cell assays with other in vitro models
  • Caveats and recent improvements of stem cell assays
  • Legislation, regulation
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10:00

Best Practice

Nicole zur Nieden

Nicole zur Nieden, Research Group Leader , Fraunhofer Institute For Cell Therapy & Immunology

  • choice of cell species
  • standardization of stem cell culture
  • pluripotency
  • serum-free versus serum-containing cultures
  • choosing an assay endpoint
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10:50

Morning Coffee and Poster Viewing

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11:20

Discussion

Nicole zur Nieden

Nicole zur Nieden, Research Group Leader , Fraunhofer Institute For Cell Therapy & Immunology

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12:10

Summary

Nicole zur Nieden

Nicole zur Nieden, Research Group Leader , Fraunhofer Institute For Cell Therapy & Immunology

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12:30

Close of Workshop

VENUE

Crowne Plaza Hotel - St James

Buckingham Gate 45/51, London, United Kingdom

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Crowne Plaza Hotel - St James

Buckingham Gate 45/51
London SW1E 6AF
United Kingdom

Crowne Plaza Hotel - St James

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