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Cell Based Assays and High Content Analysis
17 November 2009
Cell Based Assays and High Content Analysis

In association with:

Hosted by: Anthony Davis, Insitute of Molecular Medicine

OVERVIEW

The complexity of contemporary tasks in biomedical research has led to the rapid development of high content analysis technologies (HCA). In contrast to the more traditional cellular analysis approaches, these technologies enable the researcher to monitor and analyse multiple molecular targets in individual cells at sub-cellular resolution level in an automated multi-well format.

ABOUT THE WORKSHOP HOST

Dr Anthony Mitchell Davies has worked in cell based assay development for over a decade, he is currently the Director of the high content facility at Trinity College Dublin, where has led numerous training courses in HCS/A, including an academic masters course which has been running for the last 3 years.   Dr Davies’s personal research interests are focused on miniaturisation high content assays and development of improved environmental control systems for these cell based systems.    

ABOUT TRINITY COLLEGE DUBLIN HIGH CONTENT RESEARCH FACILITY

The Trinity College High Content Research Facility was initially set up in 2004 as part of a larger strategic plan for Trinity Colleges’ Institute of Molecular Medicine. Since its inception, the utilisation of this research facility has grown to a point where it now plays a central role in numerous research projects and collaborations which take place in both the national and international scientific arenas. Over the last four years the reputation of this facility has grown to a point where it is now considered to be an international centre of excellence for both technical expertise and advanced training. The success of this facility has been underpinned by the efforts of our dedicated team of scientific specialists, whose primary research goal is the advancement of these technologies and their applications. We service numerous research projects, which range from large scale gene silencing (siRNA) screens, to nano-biology, where our facility is engaged in study of biological interactions of a range of nano-materials which have potential therapeutic and/or diagnostic applications. We also have numerous projects in the areas of infection and immunity, cell signaling, lung, gut and prostate cancer, heart disease, drug discovery and applied computing.

 

Workshop agenda

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13:30

Registration & Coffee

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14:00

Welcome & Introductions

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14:10

The Basics of HCS/A

  • Familiarisation
  • Gain an understating of the capabilities of this technology
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    14:30

    Principles of Assay Design and Assay Development

  • Improving understanding
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    15:10

    Afternoon Tea

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    15:40

    Common Problems

  • Highlighting the common issues encountered by HCS users
  • How to solve them
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    16:20

    Trouble Shooting Table Quiz

  • Attendees will get the chance to solve a selection  HCS/A  experimental problems   
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    17:00

    Chairman’s Closing Remarks and Close of Day One

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    WHAT IS CPD?

    CPD stands for Continuing Professional Development’. It is essentially a philosophy, which maintains that in order to be effective, learning should be organised and structured. The most common definition is:

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    GLOBAL CPD

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