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Cell Based Assays
18 November - 19 November 2009
Cell Based Assays

SAE Media Group's Cell-Based Assays conference is now in its 3rd year - click here to view our 2010 event 

 

 


 
2009 Past Event Details:

Cell-based assays have developed into a rapid and cost-effective way of determining the challenges and issues in drug design and development.

Following on from the success of the inaugural event, the SAE Media Group will bring together the leading experts and professionals for their 2nd annual event on Cell-Based Assays, to discuss the most topical and relevant issues in this sector.

This year’s conference will provide delegates with the opportunity to gain a fresh insight into the various aspects of cell-based assays. With the aim of addressing topics from High Throughput and High Content screening to the use of 3D cell cultures, this event will cater for a breadth of people. With progressions and new developments in this exciting area of research, this event is a staple requirement for anyone looking to broaden their perspective and network with leading individuals in the field of cell-based assays. Particular focus with be placed on the developments in label free technologies and GPCR drug targetting.


 

Ernst H. K. Stelzer, Scientific Group Leader, European Molecular Biology Laboratory

John Gordon, Professor of Cellular Immunology, Medical Research Council Centre for Immune Regulation

Conference agenda

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13:30

Registration & Coffee

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14:00

Welcome & Introductions

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14:10

The Basics of HCS/A

  • Familiarisation
  • Gain an understating of the capabilities of this technology
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    14:30

    Principles of Assay Design and Assay Development

  • Improving understanding
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    15:10

    Afternoon Tea

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    15:40

    Common Problems

  • Highlighting the common issues encountered by HCS users
  • How to solve them
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    16:20

    Trouble Shooting Table Quiz

  • Attendees will get the chance to solve a selection  HCS/A  experimental problems   
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    17:00

    Chairman’s Closing Remarks and Close of Day One

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    8:30

    Registration & Coffee

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    9:00

    Chairman's Opening Remarks

    Darren  Cawkill

    Darren Cawkill, Senior Principal Scientist , Pfizer

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    9:10

    LABEL-FREE SCREENING FOR GPCR LIGANDS

    Clay Scott

    Clay Scott, Associate Director, Lead Generation , AstraZeneca Pharmaceuticals

  • How does it compare to traditional methods?
  • Where is it best utilized in the drug discovery process?
  • Do impedence-based and optical-based biosensors give similar results?
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    9:50

    USING HUMAN EMBRYONIC STEM CELLS FOR DRUG DISCOVERY

    Paul Andrews

    Paul Andrews, Senior Scientist, Drug Discovery Unit, University of Dundee

  • Human Embryonic Stem Cells (hESCs) now can be employed for drug discovery
  • High Content Screening ideally suited for hit identification and secondary assays
  • Screens for small molecules that influence stem cell fate have been performed successfully
  • Future hold great promise for more physiologically relevant cell models of disease
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    10:30

    Morning Coffee

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    11:00

    PRINCIPLES AND APPLICATIONS OF LABEL-FREE CELL BASED ASSAYS

    Sylvie Bailly

    Sylvie Bailly, Corning Epic® Applciation Scientist, Corning Life Sciences

  • Introduction to the Epic® system
  • Cell assays principle
  • Some applications: GPCR, cytotoxicity, ion channel
  • Compound library screening
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    11:40

    CASE STUDIES:

    James Brady

    James Brady, Director, Technical Applications , MaxCyte Inc

  • Replacing stable cell lines with transiently transfected cells
  • Advantages of large scale transfections by electroporation
  • Successful applications in cell based assays
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    12:20

    Networking Lunch

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    13:30

    THREE-DIMENSIONAL IMAGING AND THREE-DIMENSIONAL SPECIMENS

    Dr Ernst H. K. Stelzer

    Dr Ernst H. K. Stelzer, Scientific Group Leader, European Molecular Biology Laboratory

  • Light sheet based fluorescence microscopy
  • Three-dimensional cell biology
  • Novel approaches to physiologically relevant model-based life sciences
  • Drug and toxicity tests with three-dimensional cell-based assays
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    14:10

    EXPANDING THE USE OF PRIMARY CELL SYSTEMS IN HIT IDENTIFICATION AND COMPOUND PROFILING

    Steven Ludbrook

    Steven Ludbrook, Section Head, GlaxoSmithKline

  • Highly representative of the in vivo state: characteristics of specific drug targets behaving in their native form
  • Used together with animal in vivo models and human disease tissue systems to progress drug molecules to clinical trials.
  • Advances in sensitive screening technologies and enhanced diversity and availability of primary cell assay samples enable the use of primary cell assays at earlier stages in drug discovery programmes.
  • Examples of mechanistic and phenotypic assays in primary cell systems, together with an outlook for future directions
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    14:50

    Afternoon Tea

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    15:20

    CELL-BASED ASSAYS THAT DETECT MECHANISMS OF HUMAN HEPATOTOXICITY

    Katya  Tsaioun

    Katya Tsaioun, President, Apredica

  • Science and economics of drug-induced liver injury
  • Assays wih transformed or primary cells?
  • Single end point or multiparameter readout?
  • Validation data and case studies
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    16:00

    BETTER CELLS LEAD TO BETTER DATA : IMPROVING CELL LINE GENERATION

    Darren  Cawkill

    Darren Cawkill, Senior Principal Scientist , Pfizer

  • Importance of cell line development for enabling cell-based assays
  • Case study: fluorescence activated cell sorting (FACS) and automation (Cello) to generate high quality cell lines for difficult targets
  • Case study: Investment in thorough cell line characterisation is worthwhile
  • Could we replace recombinant cell lines with primary cells in our early cell-based screening?
  • Case study: Possibilities regarding assay development in primary cells
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    16:40

    OPTIMISATION OF ONCOLOGY CELL BASED SCREENING TO DRIVE EFFICIENCY

    Jon Orme

    Jon Orme, Associate Principal Scientist, AstraZeneca

  • Centralisation of cell based screening
  • Process quality - LIMS, Acoustic dispensing, automation
  • Efficiency improvements made in cell based screening - Multiplex assays, cmyc ELISA's
  • Future improvements
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    17:20

    Chairman’s Closing Remarks

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    17:30

    Drinks Reception Hosted by Cyntellect

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    19:00

    Close of Day One

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    8:30

    Registration & Coffee

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    9:00

    Chairman's Opening Remarks

    Nicholas Barnes

    Nicholas Barnes, Professor of Neuropharmacology, The University of Birmingham Medical School Medical School

    Anker Jon Hansen

    Anker Jon Hansen, Principal Scientist, Novo Nordisk

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    9:10

    HIGH CONTENT ANALYSIS IN EARLY DRUG DISCOVERY

    Philip Denner

    Philip Denner, Screening, High-Content Analysis, Bayer Schering Pharma

  • Image analysis tools
  • Mechanistic pathway analysis
  • Use of living cells in functional HCA
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    9:50

    LABEL-FREE DETECTION OF CELL ACTIVATION AND CELL ADHESION

    Anker Jon Hansen

    Anker Jon Hansen, Principal Scientist, Novo Nordisk

  • Human Embryonic Stem Cells (hESCs) now can be employed for drug discovery
  • High Content Screening ideally suited for hit identification and secondary assays
  • Screens for small molecules that influence stem cell fate have been performed successfully
  • Future hold great promise for more physiologically relevant cell models of disease
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    10:30

    Morning Coffee

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    11:00

    THE CELLKEY™ SYSTEM

    Johannes Pschorr

    Johannes Pschorr, European Application Scientist, MDS Analytical Technologies

  • Principles of Cellular Dielectric Spectroscopy and impedance-based measurements
  • Analysis of signal transduction pathways of receptors
  • Compound library screening and pharmacological profiling
  • Case study: demonstrating biorelevance of the CellKey™ assay technology
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    11:40

    MODELLING THE HUMAN IMMUNE RESPONSE

    John  Gordon

    John Gordon, Professor of Cellular Immunology, Medical Research Council Centre for Immune Regulation

  • The need for HUMAN immuno-profiling
  • Unfortunately mice can lie
  • Central players (the immune cell subsets and their procurement)
  • Reconstituting the human immune response ex vivo
  • Who benefits? (Answer = Patients & Industry)
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    12:20

    Networking Lunch

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    13:50

    SIGNALLING PATHWAY-SELECTIVE GPCR ACTIVATION

    Klaus Mohr

    Klaus Mohr, Pharmacology & Toxicology Section, University Of Bonn

  • Signalling-dependent cellular dynamic mass redistribution
  • Real-time measurement by an optical biosensor technique
  • Case study: Allosteric/orthosteric GPCR activators – fine-tuning efficacy and signalling
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    14:30

    MULTIDIMENSIONAL FLUORESCENCE IMAGING

    Paul French

    Paul French, Head of Photonics Group, Imperial College London

  • Fluorescence lifetime imaging
  • Spectrally-resolved and polarisation-resolved imaging
  • Label-free contrast of diseased tissue
  • Imaging local fluorophore environment
  • High speed FLIM and FRET
  • Multiplexed FRET
  • tomoFLIM and tomoFRET – applied to optical projection tomography and fluorescence molecular tomography
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    15:10

    Afternoon Tea

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    15:40

    TARGETING WNT SIGNALLING IN CANCER

  • Development of peptide ligands that bind PDZ domain of Dishevelled
  • Molecular recognition of peptides by Dishevelled protein
  • Inhibition of Dishevelled function in cell-based assays
  • Future prospects and usage of peptide ligands as tools for small molecule discovery
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    16:20

    Chairman’s Closing Remarks

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    16:30

    Close of Conference

    Workshops

    Cell Based Assays and High Content Analysis

    Cell Based Assays and High Content Analysis

    Crowne Plaza Hotel - St James
    17 November 2009
    London, United Kingdom

    Crowne Plaza Hotel - St James

    Buckingham Gate 45/51
    London SW1E 6AF
    United Kingdom

    Crowne Plaza Hotel - St James

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    WHAT IS CPD?

    CPD stands for Continuing Professional Development’. It is essentially a philosophy, which maintains that in order to be effective, learning should be organised and structured. The most common definition is:

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